Murein hydrolase activity of surface layer proteins from Lactobacillus acidophilus against Escherichia coli

Meng Jun Gao Shu-Ming Zhang Qiu-Xiang Lu Rongrong · 2015

阅读量：50

嗜酸乳杆菌

期刊名称：

International Journal of Biological Macromolecules 2015 年 79 卷

发表日期：

2015.08.01

摘要：

The aim of this study was to investigate the murein hydrolase activities of the surface layer proteins (SLPs) from two strains of Lactobacillus acidophilus using zymography. The influence of these hydrolase activities on Escherichia coli ATCC 43893 was also evaluated by analysing their growth curve, cell morphology and physiological state. After the incubation of E. coli with SLPs, growth was inhibited, the number of viable cells was significantly reduced, examination by transmission electron microscopy showed that the cell wall was damaged and flow cytometry results indicated that the majority of the cells were sublethally injured. All of these results suggested that the SLPs of both L. acidophilus strains possessed murein hydrolase activities that were sublethal to E. coli cells.