Immunopharmacology and Immunotoxicology   2016 年 38 卷 5 期

2016.09.02

Context: Diabetes mellitus is one of the most common causes of chronic renal failure. Immunosuppressive efficacies of glucocorticoids, calcineurin inhibitors, and mycophenolic acid are possibly affected by insulin after renal transplantation in these patients. Objectives: We investigated the effects of insulin on responses of mitogen-activated human peripheral blood mononuclear cells (PBMCs) to several immunosuppressive drugs. Materials and methods: Antiproliferative efficacies of prednisolone, hydrocortisone, cyclosporine, tacrolimus, and mycophenolic acid against concanavalin A-stimulated PBMCs were evaluated in the presence of physiological (5 ??units/mL) and super physiological (50 ??units/mL) concentrations of insulin. Insulin-receptor expressions on PBMCs were evaluated by flow cytometry. Results: Insulin itself had no effects on the mitogen-induced proliferation of PBMCs. The IC