Selective separation and identification of metabolite groups of Polygonum cuspidatum extract in rat plasma using dispersion solid-phase extraction by magnetic molecularly imprinted polymers coupled with LC/Q-TOF-MS

Yang Zaiyue Cai Qizhi Chen Ning Zhou Xuemin Hong Junli · 2016

阅读量：54

期刊名称：

RSC Advances 2016 年 6 卷 15 期

发表日期：

2016

摘要：

In this work, magnetic molecularly imprinted polymers (MMIPs) were successfully prepared for specific recognition and selective enrichment of metabolite groups of Polygonum cuspidatum extract in rat plasma. Two kinds of MMIPs were obtained using polydatin (PD) and emodin-8-O-??-d-glucoside (EG) as template molecules, respectively, methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker and 2,2???-azobisisobutyronitrile as initiator. The membrane thickness of both MMIPs was approximately 10 nm. With the dendritic-grafting modification, magnetic nanoparticles (MNPs) possessed the outstretched branched structure, orderly open cavities and abundant functional groups with a good specific surface area (47.99 m