Characterization of a Novel α- L -Arabinofuranosidase from Ruminococcus albus 7 and Rational Design for Its Thermostability
Yang Ying
Sun Jiaqi
Wu Junjie
Zhang Lu-Jia
Du Lei
Matsukawa Shingo
Xie Jingli
Wei Dongzhi
· 2016
期刊名称：
Journal of Agricultural and Food Chemistry
2016 年
64 卷
40 期
摘要：
An ?±-l-arabinofuranosidase (Abf) encoding gene was obtained via genomic mining from a Ruminococcus albus strain. The specific activity of this GH 51 Abf was 73.3 U/mg at pH 6.0 and 50 ?°C. The modification of Abf, aimed at improving thermostability, was performed through different strategies. Structure-based rational design using the PoPMuSiC and the Enzyme Thermal Stability System (ETSS) predicted thermal stability of Abf and enhanced the half-life of thermal inactivation (t
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