tRNA modification profiles of the fast-proliferating cancer cells
Dong Chao
Niu Leilei
Song Wei
Xiong Xin
Zhang Xianhua
Zhang Zhenxi
Yang Yi
Yi Fan
Zhan Jun
Zhang Hongquan
Yang Zhenjun
Zhang Lihe
Zhai Suodi
Li Hua
Ye Min
Du Quan
· 2016
期刊名称：
Biochemical and Biophysical Research Communications
2016 年
476 卷
4 期
摘要：
Despite the recent progress in RNA modification study, a comprehensive modification profile is still lacking for mammalian cells. Using a quantitative HPLC/MS/MS assay, we present here a study where RNA modifications are examined in term of the major RNA species. With paired slow- and fast-proliferating cell lines, distinct RNA modification profiles are first revealed for diverse RNA species. Compared to mRNAs, increased ribose and nucleobase modifications are shown for the highly-structured tRNAs and rRNAs, lending support to their contribution to the formation of high-order structures. This study also reveals a dynamic tRNA modification profile in the fast-proliferating cells. In addition to cultured cells, this unique tRNA profile has been further confirmed with endometrial cancers and their adjacent normal tissues. Taken together, the results indicate that tRNA is a actively regulated RNA species in the fast-proliferating cancer cells, and suggest that they may play a more active role in biological process than expected.
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