Combined effects of ultra-high hydrostatic pressure and mild heat on the inactivation of Bacillus subtilis
Meng Jun
Gong Yi
Qian Ping
Yu Jian-Yong
Zhang Xiao-Juan
Lu Rongrong
· 2016
期刊名称:
LWT - Food Science and Technology
2016 年
68 卷
摘要:
Effects of combined ultra-high hydrostatic pressure and mild heat (HPMH) treatments on the food-borne microorganism Bacillus subtilis were evaluated in this study. The B. subtilis culture (counted spores less than 2 log [CFU/mL]) was subjected to 100-500 MPa at 40 ?°C-60 ?°C for 15 min at pH 7.0. Treatment with HPMH increased membrane permeability by 10%-89% as determined by the uptake of propidium iodide. Changes in membrane lipids, proteins and DNA were detected in regions 3000 to 2800 cm