A Fluorescence Immunochromatographic Assay Using Europium (III) Chelate Microparticles for Rapid, Quantitative and Sensitive Detection of Creatine Kinase MB

Lai Xiao-Hong Liang Rong-Liang Liu Tian-Cai Dong Zhi-Ning Wu Yingsong Li Linhai · 2016

阅读量：159

急性心肌梗死

肌酸激酶MB

铕（III）螯合微粒

荧光免疫层析

期刊名称：

Journal of Fluorescence 2016 年 26 卷 3 期

发表日期：

2016.05.01

摘要：

The isoenzyme creatine kinase MB is very important for diagnosis of acute myocardial infarction (AMI). Some CK-MB immunoassays are sensitive, accurate and available for clinical application, but they are expensive and time-consuming procedures. Furthermore, conventional fluorescence immunochromatographic assays (FL-ICAs) have suffered from background fluorescence interference and low analytical sensitivity. A rapid and simple FL-ICA with Eu (III) chelate polystyrene microparticles was developed to determine CK-MB in 50uL serum samples using a portable test strip reader by measuring the fluorescence peak heights of the test line (H