International Journal of Clinical and Experimental Medicine   2016 年 9 卷 2 期

2016.02.29

Objective: This study aimed to investigate the effect of miRNA-4804 on the proliferation and cytokine secretion function of human monocytes by targeting chemokine-like receptor 1 (CMKLR1). Methods: The expression levels of miRNA-4804 and cytokine mRNA in human monocytic cell line (THP1) were detected by real-time quantitative PCR. THP1 cell proliferation and surface molecules were detected by CCK8 and flow cytometry. The expression of cytokines in the cell culture supernatant was detected by enzyme-linked immunosorbent assay. The regulatory effect of miRNA-4804 on the candidate target gene CMKLR1 was determined by dual-luciferase reporter assay. Western blot was used to detect the expression of CMKLR1 protein. Results: Compared with the control group, the proliferation and expression of HLA-DR and CD86 molecules of THP1 cell induced with IFN-?? were significantly inhibited by miRNA-4804 mimics and significantly enhanced by miRNA-4804 inhibitors. The expression of IL-6, TNF-?±, and IL-12 significantly increased, whereas IL-10 expression decreased in the culture supernatant of THP-1 cells upon IFN-?? stimulation. This alteration in cytokine expression could be antagonistic and promoted after treatment with miRNA-4804 mimics and miRNA-4804 inhibitors. Dual-luciferase reporter assay and Western blot showed that miRNA-4804 inhibited CMKLR1 expression. The expression of CMKLR1 mRNA was inhibited by miRNA-4804 in the THP1 cell line. Conclusion: MiRNA-4804 could regulate the proliferation and cytokine secretion of monocytes by targeting CMKLR1, and it may be involved in the regulation of immune homeostasis in chronic hepatitis B.