Regulation of B7H6 expression on human cancer cells in response to tumor-therapy treatments
摘要：
Backgroud:The role of NKp30 expressing natural kill(NK)cells in tumor surveillance is now well established.B7H6is the only reported transmembrane NKp30 ligand that is exclusively expressed on tumor cells under steady state and its interaction with NKp30 prompts anti-tumor effect of nature killer cells.Nonetheless,regulation of B7H6 expression on cancer cells is inadequately understood.Aim:We aim to investigate the expression and regulation of B7H6 on tumor cells lines in this study.Methods:And real-time PCR was used to analyze the relative expression of B7H6 mRNA.Results:We found that B7H6 mRNA is expressed in all 12 cancer cell lines derived from leukemia,hepatocarcinoma,lung carcinoma,cervical carcinoma,ovarian carcinoma and prostate carcinoma as well as the immortal kidney epithelial cell line HEK293 cell.To investigate the regulation of B7H6 expression during cancer treatments,we treated HEK293cell line with treatments or reagents that were usually applied in chemotherapy,thermal therapy and cytokine related cancer therapy.It was found that 137Cs irradiation led to an quick increase in B7H6 expression in no more than 6 hours in a dose dependent manner.Chemotherapy reagents,Cisplatin and 5-FU,promoted B7H6 expression while they led to cell apoptosis.Non-lethal Heat shock treatment,mimicking the hyperthermia therapy,led to a transient B7H6 up-regulation which last no more than 12 hours.ATRA is now applied in tumor treatments and we found it promoted B7H6 expression.Cytokines used in cancer treatments such as IFN-gamma and TNF-alpha all led to an increase in B7H6 mRNA expression.Conclusion:Together,our findings revealed a novel mechanism of tumor therapy treatments in promoting tumor elimination by increasing B7H6 expression on cancer cells.
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