Characterization of a recombinant multifunctional glycoside hydrolase family 3 β-xylosidase/α-l-Arabinofuranosidase/β-glucosidase from Cellulosimicrobium cellulans sp. 21

Yuan Ye Hu Yanbo Zhang Han Leng Jiayi Li Fan Zhao Xuesong Gao Juan Zhou Yifa · 2016

阅读量：362

纤维化纤维菌

克隆和表达

Glycoside hydrolase家族3

期刊名称：

Journal of Molecular Catalysis B: Enzymatic 2016 年 131 卷

发表日期：

2016.09.01

摘要：

A multifunctional ??-xylosidase/?±-l-Arabinofuranosidase/??-glucosidase gene (ccxyl3a) belonging to glycoside hydrolase family 3 (GH3) was cloned from Cellulosimicrobium cellulans sp. 21 and expressed in Escherichia coli BL21 (DE3). The molecular mass of recombinant CcXyl3A was estimated to be approximately 95 kDa. With p-nitrophenyl-??-d-xyloside (pNP??Xyl) as a substrate, the purified protein presented an optimal pH of 8.5 and an optimal temperature of 45 ?°C. Moreover, CcXyl3A was activated in the presence of the metals K