A long wavelength fluorescent probe for biothiols and its application in cell imaging

Li Jiawei Yin Caixia Zhang Yongbin Chao Jianbin Huo Fangjun · 2016

阅读量：117

期刊名称：

Analytical Methods 2016 年 8 卷 37 期

发表日期：

2016.10.07

摘要：

A long wavelength and turn-on fluorescent probe (1) containing the maleimide group as a reactive site was designed and synthesized. A series of sensing studies showed that probe 1 was highly selective and sensitive with fast responses towards bio-thiols over non-thiolated amino acids in DMSO:HEPES = 1:1 (v/v, pH 7.4) solution. Furthermore, the detection limit was as low as 0.015 ??M. More importantly, the potential application of probe 1 as a biosensor for thiols was demonstrated by employing it in fluorescence imaging of Cys in HepG2 cells.