High-level expression and biochemical characterization of a novel cold-active lipase from Rhizomucor endophyticus

Duan Xiaojie Zheng Mingming Liu Yu Jiang Zhengqiang Yang Shaoqing · 2016

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低温脂肪酶

期刊名称：

Biotechnology Letters 2016 年 38 卷 12 期

发表日期：

2016.12.01

摘要：

Objectives: To identify novel cold-active lipases from fungal sources and improve their production by heterologous expression in Pichia pastoris. Results: A novel cold-active lipase gene (ReLipB) from Rhizomucor endophyticus was cloned. ReLipB was expressed at a high level in Pichia pastoris using high cell-density fermentation in a 5-l fermentor with the highest lipase activity of 1395??U/ml. The recombinant lipase (RelipB) was purified and biochemically characterized. ReLipB was most active at pH 7.5 and 25???°C. It was stable from pH 4.5??“9.0. It exhibited broad substrate specificity towards p-nitrophenyl (pNP) esters (C