Data Descriptor: Building two indica rice reference genomes with PacBio long-read and Illumina paired-end sequencing data
Zhang Jianwei
Chen Ling-Ling
Sun Shuai
Kudrna Dave
Copetti Dario
Li Weiming
Mu Ting
Jiao Wen-Biao
Xing Feng
Lee Seunghee
Talag Jayson
Song Jia-Ming
Du Bogu
Xie Weibo
Luo Meizhong
Maldonado Carlos Ernesto
Goicoechea Jose Luis
Xiong Lizhong
Wu Changyin
Xing Yongzhong
Zhou Dao-Xiu
Yu S.B.
Zhao Yu
Wang Gongwei
Yu Yeisoo
Luo Yijie
Hurtado Beatriz Elena Padilla
Danowitz Ann
Wing Rod A.
Zhang Qifa
· 2016
期刊名称：
Scientific Data
2016 年
3 卷
摘要：
Over the past 30 years, we have performed many fundamental studies on two Oryza sativa subsp. indica varieties, Zhenshan 97 (ZS97) and Minghui 63 (MH63). To improve the resolution of many of these investigations, we generated two reference-quality reference genome assemblies using the most advanced sequencing technologies. Using PacBio SMRT technology, we produced over 108 (ZS97) and 174 (MH63) Gb of raw sequence data from 166 (ZS97) and 209 (MH63) pools of BAC clones, and generated ???97 (ZS97) and ???74 (MH63) Gb of paired-end whole-genome shotgun (WGS) sequence data with Illumina sequencing technology. With these data, we successfully assembled two platinum standard reference genomes that have been publicly released. Here we provide the full sets of raw data used to generate these two reference genome assemblies. These data sets can be used to test new programs for better genome assembly and annotation, aid in the discovery of new insights into genome structure, function, and evolution, and help to provide essential support to biological research in general.
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