Genome-wide analysis of DNA methylation and cigarette smoking in a Chinese population
Zhu Xiaoyan
Li Jun
Deng Siyun
Yu Kuai
Liu Xuezhen
Deng Qifei
Sun Huizhen
Zhang Xiaomin
He Meian
Guo Huan
Chen Weihong
Yuan Jing
Zhang Bing
Kuang Dan
He Xiaosheng
Bai Yansen
Han Xu
Liu Bing
Li Xiaoliang
Yang Liangle
Jiang Haijing
Zhang Yizhi
Hu Jie
Cheng Longxian
Luo Xiaoting
Mei Wenhua
Zhou Zhiming
Sun Shunchang
Zhang Liyun
Liu Chuanyao
Guo Yanjun
Zhang Zhihong
Hu Frank B.
Liang Liming
Wu Tangchun
· 2016
期刊名称：
Environmental Health Perspectives
2016 年
124 卷
7 期
摘要：
Background: Smoking is a risk factor for many human diseases. DNA methylation has been related to smoking, but genome-wide methylation data for smoking in Chinese populations is limited. oBjectives: We aimed to investigate epigenome-wide methylation in relation to smoking in a Chinese population. Methods: We measured the methylation levels at > 485,000 CpG sites (CpGs) in DNA from leukocytes using a methylation array and conducted a genome-wide meta-analysis of DNA methylation and smoking in a total of 596 Chinese participants. We further evaluated the associations of smoking-related CpGs with internal polycyclic aromatic hydrocarbon (PAH) biomarkers and their correlations with the expression of corresponding genes. results: We identified 318 CpGs whose methylation levels were associated with smoking at a genome-wide significance level (false discovery rate < 0.05), among which 161 CpGs annotated to 123 genes were not associated with smoking in recent studies of Europeans and African Americans. Of these smoking-related CpGs, methylation levels at 80 CpGs showed significant correlations with the expression of corresponding genes (including RUNX3, IL6R, PTAFR, ANKRD11, CEP135 and CDH23), and methylation at 15 CpGs was significantly associated with urinary 2-hydroxy-naphthalene, the most representative internal monohydroxy-PAH biomarker for smoking. conclusion: We identified DNA methylation markers associated with smoking in a Chinese population, including some markers that were also correlated with gene expression. Exposure to naphthalene, a byproduct of tobacco smoke, may contribute to smoking-related methylation.
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