Sensors and Actuators, B: Chemical   2016 年 237 卷

2016.12.01

Developing effective methods to specifically detect and image cysteine in vivo has become an increasingly important area of research. Here, we synthesized and characterized two isomers of 1,8-Naphthalimide derivatives (1 and 2), and result showed 1 is a fluorescent probe for selectively detecting cysteine over other relevant biological species based on Michael addition reaction. 1 can detect cysteine by fluorescence spectrometry with a detection limit of 0.064????mol/L and can be used for detection of cysteine in living cells. The Michael addition mechanism of the reaction between 1 and cysteine was confirmed by MS and NMR spectra.